Skip to main content
PMC home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1991 Jan;87(1):347–351. doi: 10.1172/JCI114992

Insulin-like growth factor I gene expression in isolated rat renal collecting duct is stimulated by epidermal growth factor.

S A Rogers 1, S B Miller 1, M R Hammerman 1
PMCID: PMC295059  PMID: 1985106

Abstract

The renal collecting duct is a site of insulin-like growth factor I (IGF I) synthesis. Epidermal growth factor (EGF) is also synthesized within the kidney in the thick ascending limb of Henle's loop and the distal tubule. EGF has been shown to regulate IGF I expression in nonrenal tissues. To shed light upon a role of EGF in intrarenal regulation of IGF I gene expression, plasma membranes prepared from collecting ducts isolated from rat kidney and collecting ducts themselves were incubated in the presence and absence of recombinant human EGF (hEGF). hEGF enhanced phospholipase C activity in collecting duct plasma membranes establishing the potential for EGF signal transduction at this site. Inclusion of hEGF in suspensions of collecting ducts increased production of immunoreactive IGF I in a concentration-dependent manner. Production was stimulated significantly by addition of 10(-8) or 10(-6) M hEGF to suspensions for 2 h. Levels of IGF I mRNA in collecting ducts were increased 2.8-fold after incubation with 10(-6) M hEGF in vitro. Our findings demonstrate a direct action of hEGF to enhance collecting duct IGF I gene expression in vitro. Such enhancement is likely to reflect an effect of EGF to stimulate IGF I production in the collecting duct of the intact kidney. Since EGF is produced in kidney, our findings are consistent with intrarenal paracrine regulation of IGF I gene expression by EGF.

Full text

PDF
347

Images in this article

349Image
on p.349
349Image
on p.349
349Image
on p.349

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andersson G. L., Skottner A., Jennische E. Immunocytochemical and biochemical localization of insulin-like growth factor I in the kidney of rats before and after uninephrectomy. Acta Endocrinol (Copenh) 1988 Dec;119(4):555–560. doi: 10.1530/acta.0.1190555. [DOI] [PubMed] [Google Scholar]
  2. Andersson G., Jennische E. IGF-I immunoreactivity is expressed by regenerating renal tubular cells after ischaemic injury in the rat. Acta Physiol Scand. 1988 Apr;132(4):453–457. doi: 10.1111/j.1748-1716.1988.tb08352.x. [DOI] [PubMed] [Google Scholar]
  3. Bach L. A., Jerums G. Effect of puberty on initial kidney growth and rise in kidney IGF-I in diabetic rats. Diabetes. 1990 May;39(5):557–562. doi: 10.2337/diab.39.5.557. [DOI] [PubMed] [Google Scholar]
  4. Bell G. I., Fong N. M., Stempien M. M., Wormsted M. A., Caput D., Ku L. L., Urdea M. S., Rall L. B., Sanchez-Pescador R. Human epidermal growth factor precursor: cDNA sequence, expression in vitro and gene organization. Nucleic Acids Res. 1986 Nov 11;14(21):8427–8446. doi: 10.1093/nar/14.21.8427. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bortz J. D., Rotwein P., DeVol D., Bechtel P. J., Hansen V. A., Hammerman M. R. Focal expression of insulin-like growth factor I in rat kidney collecting duct. J Cell Biol. 1988 Aug;107(2):811–819. doi: 10.1083/jcb.107.2.811. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Breyer M. D., Jacobson H. R., Breyer J. A. Epidermal growth factor inhibits the hydroosmotic effect of vasopressin in the isolated perfused rabbit cortical collecting tubule. J Clin Invest. 1988 Oct;82(4):1313–1320. doi: 10.1172/JCI113732. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Carpenter G., Cohen S. Epidermal growth factor. J Biol Chem. 1990 May 15;265(14):7709–7712. [PubMed] [Google Scholar]
  8. Clemmons D. R. Multiple hormones stimulate the production of somatomedin by cultured human fibroblasts. J Clin Endocrinol Metab. 1984 May;58(5):850–856. doi: 10.1210/jcem-58-5-850. [DOI] [PubMed] [Google Scholar]
  9. Fisher D. A., Salido E. C., Barajas L. Epidermal growth factor and the kidney. Annu Rev Physiol. 1989;51:67–80. doi: 10.1146/annurev.ph.51.030189.000435. [DOI] [PubMed] [Google Scholar]
  10. Flyvbjerg A., Thorlacius-Ussing O., Naeraa R., Ingerslev J., Orskov H. Kidney tissue somatomedin C and initial renal growth in diabetic and uninephrectomized rats. Diabetologia. 1988 May;31(5):310–314. doi: 10.1007/BF00277413. [DOI] [PubMed] [Google Scholar]
  11. Hammerman M. R. The growth hormone-insulin-like growth factor axis in kidney. Am J Physiol. 1989 Oct;257(4 Pt 2):F503–F514. doi: 10.1152/ajprenal.1989.257.4.F503. [DOI] [PubMed] [Google Scholar]
  12. Humes H. D., Cieslinski D. A., Coimbra T. M., Messana J. M., Galvao C. Epidermal growth factor enhances renal tubule cell regeneration and repair and accelerates the recovery of renal function in postischemic acute renal failure. J Clin Invest. 1989 Dec;84(6):1757–1761. doi: 10.1172/JCI114359. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Jennische E., Andersson G., Hansson H. A. Epidermal growth factor is expressed by cells in the distal tubules during postnephrectomy renal growth. Acta Physiol Scand. 1987 Mar;129(3):449–450. doi: 10.1111/j.1365-201x.1987.tb10616.x. [DOI] [PubMed] [Google Scholar]
  14. Kasselberg A. G., Orth D. N., Gray M. E., Stahlman M. T. Immunocytochemical localization of human epidermal growth factor/urogastrone in several human tissues. J Histochem Cytochem. 1985 Apr;33(4):315–322. doi: 10.1177/33.4.3884705. [DOI] [PubMed] [Google Scholar]
  15. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  16. Lajara R., Rotwein P., Bortz J. D., Hansen V. A., Sadow J. L., Betts C. R., Rogers S. A., Hammerman M. R. Dual regulation of insulin-like growth factor I expression during renal hypertrophy. Am J Physiol. 1989 Aug;257(2 Pt 2):F252–F261. doi: 10.1152/ajprenal.1989.257.2.F252. [DOI] [PubMed] [Google Scholar]
  17. Miller S. B., Rotwein P., Bortz J. D., Bechtel P. J., Hansen V. A., Rogers S. A., Hammerman M. R. Renal expression of IGF I in hypersomatotropic states. Am J Physiol. 1990 Aug;259(2 Pt 2):F251–F257. doi: 10.1152/ajprenal.1990.259.2.F251. [DOI] [PubMed] [Google Scholar]
  18. Norman J., Tsau Y. K., Bacay A., Fine L. G. Epidermal growth factor accelerates functional recovery from ischaemic acute tubular necrosis in the rat: role of the epidermal growth factor receptor. Clin Sci (Lond) 1990 May;78(5):445–450. doi: 10.1042/cs0780445. [DOI] [PubMed] [Google Scholar]
  19. Rall L. B., Scott J., Bell G. I., Crawford R. J., Penschow J. D., Niall H. D., Coghlan J. P. Mouse prepro-epidermal growth factor synthesis by the kidney and other tissues. Nature. 1985 Jan 17;313(5999):228–231. doi: 10.1038/313228a0. [DOI] [PubMed] [Google Scholar]
  20. Rogers S. A., Hammerman M. R. Insulin-like growth factor II stimulates production of inositol trisphosphate in proximal tubular basolateral membranes from canine kidney. Proc Natl Acad Sci U S A. 1988 Jun;85(11):4037–4041. doi: 10.1073/pnas.85.11.4037. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Rogers S. A., Miller S. B., Hammerman M. R. Growth hormone stimulates IGF I gene expression in isolated rat renal collecting duct. Am J Physiol. 1990 Sep;259(3 Pt 2):F474–F479. doi: 10.1152/ajprenal.1990.259.3.F474. [DOI] [PubMed] [Google Scholar]
  22. Safirstein R., Zelent A. Z., Price P. M. Reduced renal prepro-epidermal growth factor mRNA and decreased EGF excretion in ARF. Kidney Int. 1989 Nov;36(5):810–815. doi: 10.1038/ki.1989.266. [DOI] [PubMed] [Google Scholar]
  23. Stiles A. D., Sosenko I. R., D'Ercole A. J., Smith B. T. Relation of kidney tissue somatomedin-C/insulin-like growth factor I to postnephrectomy renal growth in the rat. Endocrinology. 1985 Dec;117(6):2397–2401. doi: 10.1210/endo-117-6-2397. [DOI] [PubMed] [Google Scholar]
  24. Teitelbaum I., Strasheim A., Berl T. Epidermal growth factor-stimulated phosphoinositide hydrolysis in cultured rat inner medullary collecting tubule cells. Regulation by G protein, calcium, and protein kinase C. J Clin Invest. 1990 Apr;85(4):1044–1050. doi: 10.1172/JCI114534. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Vehaskari V. M., Hering-Smith K. S., Moskowitz D. W., Weiner I. D., Hamm L. L. Effect of epidermal growth factor on sodium transport in the cortical collecting tubule. Am J Physiol. 1989 May;256(5 Pt 2):F803–F809. doi: 10.1152/ajprenal.1989.256.5.F803. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES