FIG. 1.

Wild-type 2G12 is heavily somatically mutated. The germ line 2G12 amino acid sequence was derived from the original nucleotide sequence using IMGT/V-QUEST (6). The wild-type 2G12 heavy chain (wt2G12H) has 38 somatic mutations, and the wild-type 2G12 light chain has 16 (wt2G12K). Residues differing from those in germ line 2G12 (gl2G12) are in boldface. Residues proposed to promote domain exchange (dx) are shaded in blue, binding/contact residues (b) are shaded in red, and infrequent residues (i) are shaded yellow (see the text). CDRs according to the Kabat definition are boxed.