FIG. 6.

No evidence for an equilibrium between domain-exchanged and conventional forms of germ line 2G12dxEA. (A) Purified Fabs were analyzed and separated in monomer and dimer configurations with SEC (shaded area). After storage at 4°C for approximately 2 weeks and incubation for 3 h at 37°C, the distribution of dimers (solid line) and monomers (dashed line) remained unchanged. (B) Expression of germ line 2G12HdxEA at 37°C (solid line) or 32°C (dashed line) does not change the percentage of domain exchange of purified Fabs. (C) Expression of germ line 2G12HdxEA at 37°C (solid dots) or 32°C (open squares) does not change the M1G1 binding of crude supernatant.