FIG. 6.

Viral polymerase cofactor function of arginine-to-lysine and lysine-to-arginine mutations for representative residues from the first basic patch, border residues, and the central basic patch. Empty vector (−), WT VP35 (VP35), or the indicated mutants were assessed for function in the minigenome assay, as described in the legend to Fig. 3. Error bars represent SDs, and corresponding Western blots (WB) of cell lysates are shown below the graphs. The concentrations of VP35 plasmids used were 125 ng, 250 ng, and 500 ng. Data were expressed as the fold induction over that by the negative control (no VP35).