FIG. 5.
NF45 does not accumulate in the cytoplasm in the presence of VP3 and VP1. Either T7 polymerase in vitro-transcribed cRNA of segment A (Seg A) of IBDV strain D78 or recombinant plasmids encoding IBDV VP1 and VP3 (VP1/VP3) were transfected into DF-1 cells. At 12 h p.t., the cells were ethanol fixed and incubated with mouse anti-VP3 MAb IBDV2 (VP3), chicken anti-VP1 serum (VP1), or rabbit anti-ckNF45-His serum (NF45). The DNA in the cellular nuclei was stained with DAPI. The presence of the primary antibody was detected with goat anti-mouse-Cy5, goat anti-rabbit-FITC, and goat anti-chicken-Cy3. The pictures were taken by confocal laser scanning microscopy and were also merged.