FIG. 6.

Blocking of Crm1-dependent export does not influence cytoplasmic accumulation of NF45 in the cytoplasm of infected cells. DF-1 cells were infected with low-pathogenicity avian influenza virus H5N2 or IBDV strain D78. Cells were either treated with LMB (wLMB) or left untreated (w/oLMB). At 12 h p.i., cells were ethanol fixed. Indirect immunofluorescence of H5N2-infected cells was performed using a rabbit anti-NP antiserum (NP) and a mouse anti-M1 MAb (M1) (lower panels). IBDV-infected cells were incubated with the mouse anti-VP3 MAb IBDV2 and rabbit anti-ckNF45-His serum (upper panels). The binding of the antibodies was visualized by using goat anti-mouse-Cy5 and goat anti-rabbit-FITC conjugated antibodies. The nucleus was detected by PI staining. Colocalization was visualized by merging the pictures taken by confocal laser scanning microscopy.