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. Author manuscript; available in PMC: 2010 Oct 6.
Published in final edited form as: Nat Struct Mol Biol. 2005 Dec 20;13(1):13–21. doi: 10.1038/nsmb1041

Figure 1.

Figure 1

A→I RNA editing of pri-miR RNAs by ADAR. (a) TLC analysis of pri-miRNAs edited in vitro by a mixture of recombinant ADAR1p110 and ADAR2 proteins (rADAR1p110 and rADAR2, 20 ng each). (b) A→I editing sites of four pri-miRNAs. Red, edited adenosine residues (numbered by position with the 5′ end of the mature miRNA sequence counted as +1); green, the region to be processed into the mature miRNA; arrows in pri–miR-142 hairpin structure, cleavage sites for Drosha and Dicer. (c) A quantitative summary of the editing patterns revealed by sequencing of RT-PCR cDNA clones corresponding to pri–miR-142 RNAs edited in vitro by rADAR1p150, rADAR1p110 or rADAR2. Editing frequency is represented as a percentage (number of independent cDNA clones representing the edited pri–miR-142 sequence at that site divided by the total number of cDNA clones examined).