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. Author manuscript; available in PMC: 2011 Mar 5.
Published in final edited form as: Biochem Biophys Res Commun. 2010 Feb 1;393(2):259–263. doi: 10.1016/j.bbrc.2010.01.115

Figure 3. Phosphorylation of S225 is essential for chromosome congression.

Figure 3

HeLa cells were transfected with a control or a previously characterized siRNA targeting the 3′ untranslated region of DDA3 [6]. Twenty-four hours later, cells were transfected again with GFP-DDA3 (wild type or mutants). At 54 hours post-transfection of siRNAs, cells were fixed and stained. Shown in (A) are maximum projections from deconvolved z stacks of cells stained for GFP (green), β-tubulin (red), and DNA (blue). These images are from representative cells analyzed in three independent repeat experiments. The percentage of metaphase cells with unaligned chromosomes over total metaphase cells were quantified and plotted (n=100 cells from three independent experiments) (B). As a control, the degree of DDA3-knockdown was analyzed in GFP-transfected cells by Western blotting against DDA3 and p38MAPK (a loading control) (C).