Table 2. Phosphopeptide Mapping of Dok1a.
| phosphosite(s) | modified peptide(s) | localization score (SLoMo) | ratio H/L count | ratio H/L | % variability |
|---|---|---|---|---|---|
| Peptides with reduced SILAC ratios in the presence of SU6656 | |||||
| Ser48 | LEFFDHKGSpSSGGGR | 25.6 | 2 | 0.377 | 3.308 |
| Tyr296 | LPSPPGPQELLDSPPALpYAEPLDSLR | 75 | 3 | 0.136 | 108.796 |
| Tyr337 | KKPLpYWDLYEHAQQQLLK | 62.1 | 2 | 0.033 | 38.622 |
| Tyr341 | KKPLYWDLpYEHAQQQLLKKPLYWDLpYEHAQQQLLK | 100.4 | 10 | 0.018 | 76.261 |
| Tyr362 | EDPIpYDEPEGLAPVPPQGLYDLPRLTDPKEDPIpYDEPEGLAPVPPQGLYDLPR | 142.5 | 9 | 0.198 | 16.081 |
| Tyr377 | EDPIYDEPEGLAPVPPQGLpYDLPRLTDPKEDPIYDEPEGLAPVPPQGLpYDLPR | 185.5 | 5 | 0.099 | 142.837 |
| Tyr398 | VKEEGpYELPYNPATDDYAVPPPR | 30.6 | 1 | 0.084 | |
| Tyr409 | EEGYELPYNPATDDpYAVPPPRVKEEGYELPYNPATDDpYAVPPPR | 79.6 | 20 | 0.142 | 43.88 |
| Tyr449 | SHNSALpYSQVQK | 72.1 | 21 | 0.167 | 118.322 |
| Tyr337;Tyr341 | KPLpYWDLpYEHAQQQLLK | One possibility | 1 | 0.071 | |
| Tyr362;Tyr377 | LTDPKEDPIpYDEPEGLAPVPPQGLpYDLPR | 18.6 | 4 | 0.030 | 60.981 |
| Tyr398;Tyr409 | VKEEGpYELPYNPATDDpYAVPPPR | 34.1 | 3 | 0.024 | 23.142 |
| Peptides with unchanged SILAC ratios in the presence of SU6656 | |||||
| Ser269 | ADpSHEGEVAEGKAGQGHDVLRADpSHEGEVAEGK | One possibility | 11 | 0.880 | 15.969 |
| Ser281 | LPpSPPGPQELLDSPPALYAEPLDSLR | 39.7 | 5 | 0.772 | 30.703 |
| Ser291 | LPSPPGPQELLDpSPPALYAEPLDSLR | 84.2 | 8 | 0.899 | 33.448 |
a
SLoMo score = −10 × (log(p). A score of 19 corresponds to a localization confidence with p = 0.0126.(33)