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. 2010 Jun 24;299(3):G643–G651. doi: 10.1152/ajpgi.00515.2009

Fig. 5.

Fig. 5.

Effects of HNF-4α small-interfering RNA (siRNA) transfection on the epithelial barrier of Caco-2 cells. Caco-2 cells were transfected with human HNF-4α siRNA or control siRNA on day 21 for 24 h. A: immunoblot analysis of HNF-4α protein levels. The immunoblot bands were quantified by densitometry analysis. B: immunocytochemical localization of HNF-4α. The nuclei were counterstained by propidium iodide (PI). C: epithelial barrier function. The epithelial barrier function of insert-cultured Caco-2 cells was assessed by measuring the transepithelial electrical resistance (TEER) with an epithelial voltohm-meter and the paracellular FD-4 penetration from the apical to the basolateral compartments. Results are means ± SD (n = 3 in A; n = 8 in C). *Significant differences (P < 0.05) between control siRNA and HNF-4α siRNA detected by t-test (A). *Significant differences (P < 0.05) between time points determined by ANOVA (C).