Fig. 2.
Collagen accumulation in PCLS treated with 25 mM ethanol and/or 4-MP up to 96 h. Rat PCLS were treated as described in materials and methods. Every 24 h, slices were lysed with RIPA as described and subjected to SDS-PAGE and immunoblotting. Collagen accumulation for each time point was normalized to t0. Tubulin was used as a loading control. Representative bands corresponding to collagen and tubulin for each time point and condition are shown below the graph. *P < 0.05; n = 5 different rat experiments.