Table 1.
Ethanol metabolism and acetaldehyde production
| Condition | Ethanol Metabolized, μmol•mg protein−1•24 h−1 | Acetaldehyde Produced, μM |
|---|---|---|
| 24 h | ||
| E | 13.64 ± 1.99 | 8.0 ± 2.0 |
| EP | 4.23 ± 0.56 | 6.0 ± 3.0 |
| 48 h | ||
| E | 12.96 ± 3.19 | 52.0 ± 24.8 |
| EP | 2.42 ± 0.52 | 0.0 ± 0.0 |
| 72 h | ||
| E | 9.61 ± 2.68 | 102.0 ± 33.6 |
| EP | 4.37 ± 1.44 | 2.0 +/−2.2 |
| 96 h | ||
| E | 9.02 ± 1.01 | 122.0 ± 44.3 |
| EP | 3.45 ± 0.75 | 4.0 ± 2.7 |
Rat precision-cut liver slices (PCLS) were cultured ≤96 h in the absence or presence of 25 mM ethanol and/or 0.5 mM 4-methylpyrazole (4-MP). E, presence of 25 mM ethanol; EP, ethanol + 0.5 mM 4-MP. Every 24 h, samples were analyzed by headspace gas chromatography for ethanol and acetaldehyde concentrations as described in materials and methods. Note that 4-MP effectively inhibited both ethanol metabolism and acetaldehyde production as observed previously (20); n = 5 different rat experiments.