Fig. 1.

Expression of calcium-regulating genes and vitamin D-metabolizing enzymes during transmissible murine colonic hyperplasia (TMCH). A: total RNA was extracted from colonic crypts isolated from either uninfected (N) or Citrobacter rodentium (CR)-infected mice 6 (D₆) and 12 (D₁₂) days postinfection. The indicated gene products were amplified by semiquantitative RT-PCR (with primers shown in Supplemental Table S1); relative changes in TRPV6 mRNA are shown at bottom. Total RNA was extracted from either kidneys (B) or colonic crypts (C) of uninfected or infected mice; GAPDH mRNA was used as a loading control. D: CYP27B1 protein was assessed in tissue extracts from isolated crypts or whole distal colon homogenates by Western blotting and β-actin was used as loading control. Results shown are representative of 3 experiments each performed with 2–4 mice per treatment group. A white line was inserted in C to indicate the joining of separate fields from a single gel image.