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. 2010 Jul 8;299(3):G707–G714. doi: 10.1152/ajpgi.00101.2010

Fig. 6.

Fig. 6.

Effect of anion channel blockers on mucosal DC-EBIO-enhanced Isc and net 86Rb fluxes in normal rat distal colon. Both basal Isc (A) and net 86Rb flux (B) were measured as described in methods. Isc and net 86Rb fluxes were also measured in presence of mucosal DC-EBIO (control) and mucosal DC-EBIO + mucosal inhibitors. The inhibitors used were 100 μM NPPB, 300 μM glibenclamide, 100 μM CFTRinh-172, 100 μM niflumic acid, Glib/Niflu, and Glib/Inh-172/Niflu. The anion channel blockers did not significantly alter the control conductance (7.9 ± 0.7). Absolute DC-EBIO-enhanced Isc and net 86Rb fluxes presented were calculated by subtracting Isc and 86Rb fluxes obtained in the absence of DC-EBIO from that in presence of DC-EBIO, respectively. Mucosal medium always contained 1 mM VO4, whereas serosal medium contained 3 mM Ba2+. Results presented represent means ± SE of 6 tissue pairs. *P < 0.001 compared with respective control; £P < 0.05 compared with respective control.