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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Cell Immunol. 2010 Aug 14;265(2):139–145. doi: 10.1016/j.cellimm.2010.08.002

Figure 1B. LPS-induced formation of new blood vessels is suppressed in the presence of morphine.

Figure 1B

Cryostat sections from nitrogen snap frozen matrigel samples were stained using nuclear (DAPI) and PECAM (CD31) markers. In placebo treated groups, LPS-induced angiogenesis was apparent throughout matrigel plugs by Day 7. Chronic morphine treatment significantly decreased the formation of blood vessels into LPS containing plugs. Skeletonized images of newly formed blood vessels were assessed to measure vessel node, length, and end points.