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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Cell Immunol. 2010 Aug 14;265(2):139–145. doi: 10.1016/j.cellimm.2010.08.002

Figure 3A. Morphine suppresses hypoxia-stabilized protein levels of HIF-1α in cytosolic and nuclear fractions.

Figure 3A

Peritoneal mouse macrophages were harvested and pretreated with 1μM morphine 16h prior to hypoxia stimulation. Cytosolic (lower gel) and nuclear (upper gel) extracts were analyzed using anti-HIF-1α monoclonal Ab. In the cytoplasmic fractions, hypoxia stabilization of HIF-1α protein was attenuated in the presence of morphine. Nuclear fractions of HIF-1α protein showed elevated levels in response to hypoxia. However, less HIF-1α was detected in the nuclear compartment following morphine treatment.