Figure 5. Capsid and endogenous reverse transcription.

(A) Virus was lysed with detergent in the presence of S100 or PGF fraction for 18 h, subjected to ultracentrifugation, the pellet was resuspended, and the amount of p24 in the pellet and supernatant fractions was measured. A no-detergent control was also included for comparison. (B) Virus capsid mutants with cores of varying stabilities were used as a source of virus in ERT reactions and their ability to generate late products with and without added PGF fraction was determined by quantitative PCR using the “full-length” primer set. The data shown are representative of at two independent experiments.