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. Author manuscript; available in PMC: 2011 Oct 7.
Published in final edited form as: Mol Cell Endocrinol. 2010 Jun 14;327(1-2):89–97. doi: 10.1016/j.mce.2010.06.006

Fig. 2.

Fig. 2

Pull-down assays to verify the UBQLN1-SPEM1 interactions in mammalian cells. FLAG-tagged SPEM1 and V5-tagged UBQLN1 were both expressed in the COS-7 cells, as shown by Western blot analyses (WB). In the immunoprecipitation (IP) assays, V5-tagged UBQLN1 was detected in the anti-FLAG immunoprecipitants, suggesting UBQLN1 indeed interacts with SPEM1 in mammalian cells.