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. Author manuscript; available in PMC: 2011 Oct 7.
Published in final edited form as: Mol Cell Endocrinol. 2010 Jun 14;327(1-2):89–97. doi: 10.1016/j.mce.2010.06.006

Fig. 3.

Fig. 3

Expression profiles of Ubqln1 mRNA and protein. (A) Northern blot analyses of Ubqln1 mRNAs in 12 mouse organs. 28S and 18S rRNA bands were shown as loading and mRNA quality controls. (B) Northern blot analyses of Ubqln1 mRNA in developing testes in mice. Testes of postnatal days 7-42 (P7-P42) were analyzed, and 28S and 18S rRNA bands were shown as loading and mRNA quality controls. (C) Levels of Ubqln1 mRNA in purified testicular cell populations determined using a semi-quantitative PCR method. The hypoxanthine-guanine phosphoribosyltransferase (Hprt) gene was used as loaning control. PCR cycle number for Ubqln1 and Hprt were 26 and 20, respectively, which were tested to be within the exponential range. (D) Western blot analyses of UBQLN1 in mouse developing testes. ACTIN was used as a loading control.