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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Nat Struct Mol Biol. 2010 Sep 19;17(10):1210–1217. doi: 10.1038/nsmb.1901

Figure 4. The primary RecBC translocase site remains bound to ssDNA upon reaching a 5′-end, while its secondary translocase continues.

Figure 4

(a) RecBC translocation along a partial duplex substrate with a 5′ to 3′ dT60 ssDNA extension, doubly labeled with Cy3 and Cy5 as shown. Time course monitoring FRET as Cy5 fluorescence (filled circles) (due to exciting Cy3 donor) shows that the 3′-terminated ssDNA remains bound to RecBC while the secondary translocase continues; × Translocation time course for the same DNA, but without the Cy5 label (opened squares). (b). RecBC translocation along a partial duplex substrate with a 3′ to 5′ dT60 ssDNA extension doubly labeled with Cy3 and Cy5 as shown. Time course monitoring FRET as Cy5 fluorescence (filled circles) (due to exciting Cy3 donor) shows dissociation of the 5′-terminated ssDNA; Translocation time course for the same DNA, but without the Cy5 label (opened squares).