Table 1.
Summary of proteomic analysis of different types of membrane fractions1.
| Fraction | 1–4 | 5 | 7 | 9 |
|---|---|---|---|---|
| Ion spectra | 284 | 1765 | 4500 | 6075 |
| Unique peptides (2 exp < 0.05) | 51 | 57 | 837 | 1247 |
| Identified proteins (unique pep ≥ 2) | 14 | 12 | 188 | 247 |
1
Membrane fractions were prepared by a non-detergent based method as described in the Materials and Methods. After confirming the quality of the partitioning by comparing the refractive index of each fraction with our previous sucrose density data [9], proteins for fractions 1-4, 5, 7 and 9 were extracted by TCA precipitation and were separated by SDS-page. Each lane was cut into 9 pieces and utilized for GeLC-MS/MS analysis after “in-gel digestion” using trypsin.
2
Mascot expectation value.