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Accuracy of pyrosequencing to quantify the two alleles of SNPs. Genomic DNA was extracted from cells homozygous for either allele of the SNP markers and quantified using NanoDrop. For each SNP, the two DNAs representing the alternative alleles were mixed at different ratios to simulate expected allele ratios. PCR was performed on each mixture, followed by pyrosequencing to determine the observed allelic ratios. Pearson’s correlation test is used to calculate the P-value.
