Figure 6.

NGFI-A binding to its response element triggers active demethylation of GR promoter. a, Mean ± SEM percentage methylation of the 5′ CpG dinucleotide for NGFI-A bound exon 1₇ GR and its respective mutants from the cotransfection shown in Figure 4 c (6–10 clones sequenced per sample; n = 4 samples per group) in the absence (control) and presence of an NGFI-A expression vector (**p < 0.001). b, Mean ± SEM percentage methylation of the 3′ CpG dinucleotide (6–10 clones sequenced per sample; n = 4 samples per group).