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Allergic sensitization, ovalbumin (OVA) challenge, and ultrafine particles (UFP) inhalation protocol. Animals were intranasally sensitized with saline, OVA, or OVA + UFP followed by inhalation exposure to filtered air (FA) or concentrated ambient UFP in the presence of 1% aerosolized OVA. Necropsy (Nec) was conducted on day 26.
