Figure 4. Cultured human olfactory cells respond to helional, a ligand for the OR3A1.

Cultured hOE cells were loaded with Fura-2/AM and imaged as described in Experimental Procedures. Stimuli were added for 10 sec, followed by a 2 minute rinse with Ringer's solution. Figure 4 depicts traces from a subset of 165 cells assayed from two experiments using cells derived from the same source as that used for in situ hybridization. Traces for one helional-responsive and three unresponsive cells are shown. Additional experiments using two independent cultures yielded similar results.