Figure 1.
CD40 induced IL-6 production following pre-stimulation with TLR agonists. A) Human peripheral B cells and B) mouse high density B cells were treated with R-848 (0.2μg/ml), anti-BCR (Fab')2 (1μg/ml), both, or medium alone for 24 hours. Cells were then washed, rested for 1 hour, and stimulated with Hi5 cell controls or Hi5 cells expressing CD154 for 20 hours. Supernatants were then collected and assayed for IL-6 by ELISA. Each graph is representative of two individual experiments.