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. Author manuscript; available in PMC: 2010 Oct 7.
Published in final edited form as: Clin Cancer Res. 2009 Jan 15;15(2):452–459. doi: 10.1158/1078-0432.CCR-08-1631

Figure 4. Effect of Notch1 knockdown on mRNA and protein expressions of uPA and uPAR.

Figure 4

(A) Semiquantitative RT-PCR analysis shows mRNA expression of uPA and uPAR in cells transfected with increasing concentration of Notch1 siRNA. Scrambled siRNA was used as control in parallel. Lower panel showing GAPDH as an internal control. (B) Western imunoblot analysis of uPAR and uPA expression in PC3 cells transfected with increasing concentration of Notch1 specific siRNA. Blots were reprobed with β-actin antibody to analyze the equal loading of proteins. (C-D) Real time RT-PCR analysis showing mRNA expression of uPA and uPAR.