Table 1.
| Table 1A. Expression of Notch1 in human normal prostate, HGPIN, and adenocarcinoma specimens. | ||||||
|---|---|---|---|---|---|---|
| Samples | Number | None | Weak | Moderate | Strong | P value |
| Normal glands | 91 | 1 (1%) | 33 (37%) | 53 (58%) | 4 (4%) | - |
| High grade PIN | 69 | 0 (0%) | 7 (10%) | 41 (60%) | 21 (30%) | 0.001* |
| Gleason pattern 3 | 130 | 0 (0%) | 5 (4%) | 57 (44%) | 68 (52%) | 0.001* |
| Gleason pattern 4 | 28 | 0 (0%) | 0 (0%) | 10 (36%) | 18 (64%) | 0.001* |
| Table 1B: List of genes modulated by knockdown of Notch1 in PC3 cells | ||||||
|---|---|---|---|---|---|---|
| List of genes downregulated by specific knockdown of Notch1 | ||||||
| UniGene | Ref Seq # | Symbol | Description | Fold change | ||
| Hs.77274 | NM_002658 | PLAU | Plaminogen activator, urokinase | 34.66 | ||
| Hs.514412 | NM_000442 | PECAM1 | Platelet/endothelial cell adhesion molecule (CD31 antigen) | 18.16 | ||
| Hs.643357 | NM_006988 | ADAMTS1 | ADAM metallopeptidase with thrombospondin type 1 motif, 1 | 11.00 | ||
| Hs.592171 | NM_002214 | ITGB8 | Integrin, beta 8 | 8.68 | ||
| Hs.632226 | NM_000213 | ITGB4 | Integrin, beta 4 | 8.12 | ||
| Hs.74034 | NM_001753 | CAV1 | Caveolin 1, caveolae protein, 22kDa | 6.81 | ||
| Hs.482077 | NM_002203 | ITGA2 | Integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor) | 6.46 | ||
| Hs.55279 | NM_002639 | SERPINB5 | Serpin peptidase inhibitor, clade B (ovalbumin), member 5 | 5.02 | ||
| Hs.58488 | NM_003798 | CTNNAL1 | Catenin (cadherin-associated protein), alpha-like 1 | 4.58 | ||
| Hs.643447 | NM_000201 | ICAM1 | Intercellular adhesion molecule 1 (CD54), human rhinovirus receptor | 4.00 | ||
| Hs.133397 | NM_000210 | ITGA6 | Intregrin, alpha 6 | 3.82 | ||
| Hs.466871 | NM_002659 | PLAUR | Plasminogen activator, urokinase receptor | 3.40 | ||
| Hs.609663 | NM_002293 | LAMC1 | Laminin, gamma 1 (formerly LAMB2) | 2.93 | ||
| Hs.371147 | NM_003247 | THBS2 | Thrombospondin 2 | 2.43 | ||
| Hs.297413 | NM_004994 | MMP9 | Matrix metallopeptidase 9 gelatinase B, 92kDa gelatinase, 92kDA type IV collagenase | 4.20 | ||
| Hs.159581 | NM_016155 | MMP17 | Matrix metallopeptidase 17 (membrane-inserted) | 2.20 | ||
| Hs.411312 | NM_000419 | ITGA2B | Integrin alpha 2b (platelet glycoprotein llb of llb/llla complex, antigen CD41) | 2.08 | ||
| List of genes upregulated by specific knockdown of Notch1 | ||||||
| Hs.313 | NM_000582 | SPP1 | Secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activator) | 9.07 | ||
| Hs.73800 | NM_003005 | SELP | Selectin P (granule membrane protein 140kDa, antigen CD62) | 6.66 | ||
| Hs.21422 | NM_005010 | NRCAM | Neuronal cell adhesion molecule | 5.07 | ||
| Hs.567417 | NM_006690 | MMP24 | Matrix metallopeptidase 24 (membrane-inserted) | 5.01 | ||
| Hs.300774 | NM_005141 | FGB | Fibrinogen beta chain | 4.38 | ||
| Hs.375129 | NM_002422 | MMP3 | Matrix metallopeptidase 3 (stromelysin 1, progelatinase) | 3.29 | ||
| Hs.652397 | NM_00362 | TIMP3 | TIMP metallopeptidase inhibitor 3 (Sorbsy fundus dystrophy, pseudoinflammatory) | 3.21 | ||
| Hs.2936 | NM_002427 | MMP13 | Matrix metallopeptidase 13 (collagenase 3) | 3.10 | ||
| Hs.161985 | NM_019894 | TMPRSS4 | Transmembrane protease, serine 4 | 2.67 | ||
| Hs.143434 | NM_001843 | CNTN1 | Contactin 1 | 2.64 | ||
| Hs.204732 | NM_021801 | MMP26 | Matrix metallopeptidase 26 | 2.60 | ||
| Hs.461086 | NM_004360 | CDH1 | Cadherin 1, type 1, E-cadherin (epithelial) | 2.50 | ||
| Hs.445981 | NM_001903 | CTNNA1 | Catenin (cadherin-associated protein), alpha 1, 102kDa | 2.16 | ||
Note. The expression of Notch-1 was evaluated as staining of the tissue as none (0), weak (1), moderate (2), and strong (3). Fisher exact test was used to examine the association between staining intensity and tissue type or staining intensity and tumor grade (for tumor specimen only).
*
P<0.001 was considered as significant.