TABLE 3.
Two-dimensional gel analysis of WT and SUB−/−Leishmania
Spots that differed in intensity between the wild-type and SUB −/− parasites were selected for peptide sequencing by mass spectrometry. Nine distinct spots were found to be tryparedoxin peroxidases, the terminal peroxidases of the trypanothione reductase system. In the L. donovani complex, this family of peroxidases is composed of three cytoplasmic tryparedoxin peroxidases, TXNPx1–3 (TRYP1–3), and a mitochondrial peroxidoxin.
| WT |
SUB −/− |
|||
|---|---|---|---|---|
| Spot | Protein | Spot | Protein | Relative to WT |
| 1 | No Prx detected | 1 | Prx | Elevated |
| 2 | Prx | Elevated | ||
| 3 | TXNPx2 | 3 | TXNPx2 | Same |
| 4 | TXNPx1/3 | 4 | TXNPx1/3 | Decreased |
| 5 | TXNPx1/3 | 5 | TXNPx1/3 | Decreased |
| 6 | TXNPx1/3 | Elevated | ||
| 7 | No TXNPx detected | 7 | TXNPx1/3 | Elevated |
| 8 | TXNPx1/3 | Elevated | ||
| 9 | TXNPx1/3 | Elevated | ||