FIGURE 3.

Reciprocal decrease in E-cadherin expression with GH-dependent increase in ZEB2 expression in glomerular podocyte. A, immortalized differentiated human podocytes were exposed to hGH (500 ng/ml) as detailed under “Experimental Procedures.” E-cadherin mRNA abundance was measured by RT-qPCR and is depicted relative to E-cadherin mRNA abundance prior to exposure to GH; GAPDH was used as an internal control. The results (n = 4–5) are depicted as mean ± S.E. *, p < 0.05 (Kruskal-Wallis test) compared with expression prior to exposure to GH. B, immortalized differentiated human podocytes were exposed to hGH (500 ng/ml) for the indicated time periods and subjected to Western blot analysis for E-cadherin and tubulin as detailed under “Experimental Procedures.” Results shown are representative of three independent experiments. C, immortalized differentiated human podocytes were exposed to the indicated concentrations of hGH for 48 h and subjected to Western blot analysis sequentially for ZEB2, E-cadherin, and tubulin as detailed under “Experimental Procedures.” Results shown are representative of three independent experiments. D, immortalized differentiated murine podocytes (MPC-5) were exposed to ovine GH (oGH) (500 ng/ml) for the indicated time periods as detailed under “Experimental Procedures.” ZEB2 mRNA abundance was measured by RT-qPCR and is depicted relative to ZEB2 mRNA abundance prior to exposure to GH; GAPDH was used as an internal control. Error bars indicate mean ± S.E.; n = 4–5. *, p < 0.05 (Kruskal-Wallis test) compared with expression prior to exposure to ovine GH. E, immortalized differentiated murine podocytes (MPC-5) were exposed to ovine GH (500 ng/ml) for the indicated time periods and then subjected to Western blot analysis sequentially for ZEB2, E-cadherin, and tubulin as detailed under “Experimental Procedures.” Results shown are representative of three independent experiments.