FIGURE 4.

GH-dependent decrease in P-cadherin expression in glomerular podocyte. A, immortalized differentiated human podocytes were exposed to hGH (500 ng/ml) for the indicated time periods as detailed under “Experimental Procedures.” P-cadherin mRNA abundance was measured by RT-qPCR and is depicted relative to P-cadherin mRNA abundance prior to exposure to GH; GAPDH was used as an internal control. Error bars indicate mean ± S.E.; n = 4–5. *, p < 0.05 (Kruskal-Wallis test) compared with expression prior to exposure to GH. B, immortalized differentiated human podocytes were exposed to hGH (500 ng/ml) for the indicated time periods and subjected to Western blot analysis for P-cadherin and tubulin as detailed under “Experimental Procedures.” HepG2 lysates were also similarly analyzed as a positive control. Results shown are representative of three independent experiments.