FIGURE 7.

ZEB2 is essential for GH-dependent increase in permeability of podocyte monolayer. Immortalized human podocytes transduced with lentiviral construct expressing ZEB2 shRNAs 1 and 2 (right), untransduced (left), or transduced with lentiviral construct expressing scrambled shRNA (left) were grown as a monolayer on collagen-coated Transwell filters and induced to differentiate for 14 days prior to incubation with vehicle (black bar), hGH (500 ng/ml) (gray bar), or TGF-β (white bar) for 48 h, and albumin permeability across the podocyte monolayer was determined at 1, 2, and 4 h following the 48-h exposure to hGH. Error bars indicate mean ± S.E.; n = 4. The data for the TGF-β experiments are depicted as the average of two experiments. *, p < 0.05 versus control (CON).