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Pyk2 tyrosine phosphorylation is not induced by Ca2+ in all Pyk2-expressing cells. ConA-activated splenic T cell blasts were stimulated with 0.5 μm thapsigargin (A) and B3Z (B), NIH-3T3 (C), Jurkat 6.8 T cells (D), RNK (E), or WEHI-231 (F) were treated with the indicated concentration of ionomycin for 10 min. Pyk2 was immunoprecipitated and probed by immunoblotting for phosphotyrosine followed by Pyk2.
