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Changes of MRP2/ABCC2 and BSEP/ABCB11 protein expression in SC human hepatocytes exposed to TNF-α, IL-6, and IL-1β. The membrane protein fraction was prepared from SC human hepatocytes from 2–3 wells of a 24-well plate and subjected to LC-MS/MS SRM analysis for absolute quantification of MRP2/ABCC2 (A) or BSEP/ABCB11 (B) protein. The data represent the mean ± S.D. (n = 3). *, statistically significant (p ≤ 0.05 by ANOVA). SRM, selected reaction monitoring.
