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. 2010 Jul 25;285(41):31253–31260. doi: 10.1074/jbc.M110.115881

FIGURE 5.

FIGURE 5.

Gel electrophoresis of yeast membrane proteins under nonreducing SDS-PAGE conditions and detection of aquaporins by specific antibodies. Membrane proteins from yeast expressing either NtAQP1-YC or NtPIP2;1 or coexpressing both genes (Co) were separated in a polyacrylamide gel and detected with a NtAQP1-specific antibody (NtAQP1) or NtPIP2;1 specific antibody (NtPIP2;1). Arrows indicate the location of additional bands in samples from yeasts coexpressing NtAQP1-YC and NtPIP2;1. The depicted bands have an estimated molecular mass of at least 120 kDa. Membrane proteins from yeast cells not expressing NtAQP1-YC or NtPIP2;1 (not transformed or no induction of expression) do not lead to a signal with the employed NtAQP1- or NtPIP2;1-specific antibodies.