FIGURE 3.

The substitution N415C slows kinetics of ASIC1 channels. Representative examples of macroscopic scaled currents recorded from oocytes with the two-electrode voltage clamp recordings from rat ASIC1a (A), Xenopus ASIC1.1 (B), and XenopusASIC1.1-M85L (C). The parent channels are shown in black, and the same channel with the mutation N415C is shown in gray. The lines over the traces indicate the duration of the activating stimulus. The activating pH was 6.5 for rASIC1a and 7.0 for xASIC1.1. Holding potential, −60 mV. The time scale is the same for all recordings and is shown by the bar on the right. D, representative examples of outside-out patches expressing xASIC1.1 wild type and with the indicated single and double mutations. Currents were evoked by changing pH from 7.6 to 7.0 for 10 s. The time scale bar is 1 s for all traces, whereas the current scale bar is 100 pA, except for M85L, which is 10 pA.