FIGURE 5.

Single channel activity of xASIC1.1-N415C. Representative example of an outside-out patch expressing xASIC1.1-N415. A, the patch was first activated by pH 6.8. The magnitude of the peak current is 2.5 nA. B, the pH was returned to 7.8 for a 4-s recovery from desensitization followed by activation by pH 7.0. C, upper two traces are the first 2 s of a continuous recording of 20-s duration. The activating pH was 7.2. In 20 sweeps of a 20-s duration, only one or two channels were open simultaneously. Bars in the right lower corner indicate the time and amplitude scales. The third trace is from xASIC1.1 wild type.