TABLE 2.
Functional characteristics of HEK293 cells transfected with the indicated amounts of EAAT2wt cDNA
| cDNAa | EC50 | Hill slope | Fmax |
|---|---|---|---|
| μg | μm | ||
| 0.5 | 24.3 ± 1.3 | 1.0 ± 0.2 | 29.5 ± 1.7 |
| 1.0 | 31.0 ± 1.5 | 1.2 ± 0.2 | 28.9 ± 1.5 |
| 2.0 | 24.7 ± 1.3 | 0.9 ± 0.2 | 31.9 ± 2.0 |
| 3.0 | 29.4 ± 1.1 | 1.2 ± 0.3 | 29.1 ± 1.6 |
| 4.0 | 20.0 ± 1.2 | 1.3 ± 0.3 | 35.0 ± 1.6 |
| 5.0 | 33.5 ± 1.2 | 1.2 ± 0.3 | 32.3 ± 0.8 |
a HEK293 cells were transfected with the amount of cDNA shown per 2 × 105 cells. The data were derived from three independent experiments conducted in triplicate; values are mean ± S.E.