FIGURE 5.

Treatment with G6 or its stilbenoid derivatives leads to HEL cell death via the intrinsic apoptotic pathway. HEL cells were treated with 25 μm of the different drugs for either 8 h (A) or 24 h (B). Bcl-xL mRNA levels were normalized to those of glyceraldehyde-3-phosphate dehydrogenase and plotted as the fold change over DMSO control. Each sample was run in duplicate. Shown is one of two sets of representative results. *, p < 0.05 with respect to DMSO. C, cells were treated with either DMSO or 25 μm of the different drugs for 24 h. The whole cell lysates were then analyzed by Western blotting with an anti-poly(ADP-ribose) polymerase (PARP) antibody. Shown is one of three sets of representative results. D, cells were treated with either DMSO or 25 μm of the different drugs for 24 h. Whole cell lysates were then serially analyzed by Western blot analysis for the indicated proteins. Shown is one of three sets of representative results.