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. 2010 Jul 30;285(41):31418–31426. doi: 10.1074/jbc.M110.116418

FIGURE 3.

FIGURE 3.

Localization of Zmynd15 mRNA and protein in the mouse testis. A–D, in situ hybridization analyses of Zmynd15 mRNA localization in the testis of adult mice. Bright (A and C) and corresponding dark (B and D) field images are shown. The lower magnification images (A and B) show that the hybridization signals (black dots in bright field images and silver grains in dark field images) are confined to the luminal and adluminal compartments, and the higher power images (C and D) reveal that the hybridization signals are over pachytene spermatocytes and spermatids. The arabic numbers stand for steps of spermatid development, and the Roman numerals indicate stages of the seminiferous epithelial cycles. Sg, spermatogonia; Sp, spermatocytes; Sd, spermatids; L, Leydig cells. Scale bars, 20 μm. E and F, ZMYND15 immunoreactivity is detected mainly in the nuclei of step 2–8 round spermatids (E and F). In step 9–11 spermatids, ZMYND15 immunoreactivity appears to shift to cytoplasm (E). The arabic numbers stand for steps of spermatid development, and Roman numerals indicate stages of the seminiferous epithelial cycles. P, pachytene spermatocytes; Pl, preleptotene spermatocytes; Sd, spermatids. Scale bars, 20 μm. G, RT-PCR analyses of expression of Cxcl16 and Zmynd15 mRNAs in purified testicular cell types. Lane M, 100-bp molecular marker. NTC, nontemplate control.