FIGURE 10.

Signaling assays in COS-7 cells expressing Gα₁₆ with wild type or mutant receptors. Left and right panels, respectively, represent C5aR and CXCR4. The cells were stimulated with 10 nm C5a (C5aR) or 100 nm CXCL12 (CXCR4). IP₃ accumulation values are normalized to the mock transfections, where each bar represents means ± S.D. of three independent experiments.