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Signaling profile of wild type and mutant C5aR receptors, co-expressed with wild type and C27R C5a in yeast. The nonfunctional mutants, such as S272A and S272T, have not been tested with C27R C5a. Vector represents the β-galactosidase activity of the engineered yeast in the absence of both receptor and ligand. Each bar represents means ± S.D. of signaling activity for three independent transformants, and data are representative of at least two independent experiments.
