FIGURE 5.

Effect of point mutations on expression profile of C5aR in yeast as assessed by Western blot. 25 μl of whole yeast lysates expressing the mutant or the wild type receptors were resolved on a 4–12% BisTris gel and detected as described under “Experimental Procedures.” The single and double asterisks, respectively, indicate the full-length and proteolytic fragments of the receptors. n/a, not applicable.