FIGURE 8.

Positional effect of N-terminal and EC3 cysteines on constitutive signaling profile of C5aR and their respective response to C5a, co-expressed in yeast. Left and right panels, respectively, illustrate the effect of S30C, S272C, S30C/S272C, and D27C, S272C, D27C/S272C on ligand-independent and -dependent signaling of constitutively active C5aR. The contrasting effect of S30C/S272C, D27C/S272C, and S272C on constitutive signaling of C5aR is noted in both panels. Vector represents the β-galactosidase activity of the engineered yeast in the absence of both receptor and ligand. Each bar represents means ± S.D. of signaling activity for three independent transformants, and data are representative of at least two independent experiments.