TABLE 2.
Analysis of receptor binding affinities in COS-7 cell membranes
The apparent Kd and Bmax values are derived from binding assays by fitting the raw data into one site homologous binding models. Values represent means ± S.D. from three independent experiments.
| Receptors | Kd | Bmax |
|---|---|---|
| nm | pmol/mg | |
| C5aR | 0.65 ± 0.07 | 8.43 ± 0.85 |
| S272C-C5aR | 3.61 ± 0.38 | 7.88 ± 0.87 |
| S30C/S272C-C5aR | 1.23 ± 0.04 | 5.08 ± 0.15 |
| N119S-C5aR | 0.22 ± 0.07 | 4.02 ± 0.52 |
| S30C/N119S/S272C-C5aR | 1.11 ± 0.03 | 7.03 ± 0.42 |
| CXCR4 | 3.32 ± 0.24 | 9.41 ± 0.72 |
| N119S-CXCR4 | 1.63 ± 0.23 | 20.86 ± 2.55 |
| C28S/N119S/C274S-CXCR4 | 13.33 ± 1.15 | 16.42 ± 1.70 |
| C28R/N119S/C274E-CXCR4 | 7.59 ± 0.20 | 16.64 ± 0.46 |