FIGURE 1.
Sequences of human vitamin K-dependent protein propeptides and their relative Kd values for carboxylase. Varying concentrations of carboxylase were incubated with 4 nm of the fluorescein-labeled propeptide for 2 h at 4 °C in 100 mm MOPS, pH 7.5, 180 mm NaCl, 3.3% glycerol, 6.3 mm DTT, 66 μm EDTA, 0.1% PC, 0.28% CHAPS, and 0.4% BSA in a final volume of 300 μl. The resulting fluorescence anisotropies were measured. The fraction of bound propeptide at different carboxylase concentrations and the Kd were determined as described under “Experimental Procedures.” The values shown are from three determinations.