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. 2010 Aug 4;285(41):31537–31547. doi: 10.1074/jbc.M109.077453

FIGURE 4.

FIGURE 4.

A, schematic representation of HIV-1 subgenomic plasmids pNL13a7 (7), pNL13a7de3, and pNL13a7di3. Nucleotide positions of deletions are indicated. B and C, Northern blots with RNA extracted from HeLa cells transfected with pNL13a7 (w), pNL13a7de3 (de3), or pNL13a7di3 (di3) in the absence or presence of CMV promoter-driven plasmids expressing SRp55 or SRp75. The blots were probed with exon 1 probe (Fig. 1A) detecting all HIV-1 mRNAs. Detected mRNAs are indicated.