TABLE 1.
Oligonucleotides used for RT-PCR and plasmid construction
| Name | Sequence (5′→3′) |
|---|---|
| BSS | ggcttgctgaagcgcgcacggcaagagg |
| BAMA | gccaaggatccgttcactaatcgaatgg |
| MLUS | ggggcccggacgcgtatcagaagactgagttacaa |
| SALA | tattctgctatgtcgacaccc |
| APAA | ggtcgacggacgcgtgccctttttcctaggggccctgc |
| dGARs | ttaggcatctcctatggcaggaacagtcagactcatcaagcttctctatc |
| dGARas | gaagcttgatgagtctgactgttcctgccataggagatgcctaaggcttt |
| Downsa4s | agtcgacctagactagagccctggaag |
| 5480s | ggtcgacaagtttgtttcataacaaaagccttag |
| sd1as | ccgaattcacgcgtaatgatctaagttcttctgatcc |
| Exon1s | ggtctctctggttagaccagat |
| Exon1as | cagtcgccgcccctcgcctct |
| Exon3s | acatagttagccctaggtgtgaatatc |
| dexon3s | agaggcgaggggcgccgaaaggtaggatctctacaatacttgg |
| dexon3as | ttgtagagatcctacctttcgccgcccctcgcctcttgcc |
| sd5as | tgctttgatagagaagcttgat |
| di3sd3as | gaattcttggtgttattaatgctgctag |
| SRp55s | aagcgcgcaccatgccacgagtctacatagga |
| SRp55RRMas | ggctcgagttaatcttcaataagcctaatatt |
| T7gars | tctaatacgactcactataggggaagaagcggagacagcgacg |
| T7garas | tgatgagctcttcgtcgctgtctccgcttc |
| AnnealGARBss/XbaS | cgcgcgaagaagcggagacagcgacgaacagctcatcagaacagtcagactcatcaagcttctctatcaaagcat |
| AnnealGARBss/XbaAS | ctagatgctttgatagagaagcttgatgagtctgactgttctgatgagctcttcgtcgctgtctccgcttcttcg |
| SRp55gsts | ggggatccatgccgcgcgtctacataggac |
| SRp55gstas | gggaattcttaatctctggaactcgacctg |
| 757s | gtcgacggtatcgatcggttgtgcgtacaaagcacacacg |
| L1AM | cgctgggcagccacaggc |
| gapdhS | aggtcggagtcaacggatttgg |
| gapdhAS | acagtcttctgggtggcagtgatg |