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Quantitative distribution of reaction intermediates in the donor half-reaction of human TKT under equilibrium conditions as estimated by 1H NMR spectroscopy after acid quench isolation
Note that the conjugate acid of the DHE-ThDP carbanion/enamine intermediate is detected by NMR spectroscopy.
| Donor substrate | C2-unsubstituted ThDP | Donor-ThDP adduct | DHE-ThDP |
|---|---|---|---|
| % | % | % | |
| X5P | 25 | 75 | <5 |
| F6P | 16 | 72 | 12 |
| S7P | 10 | 50 | 40 |
