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Interactions between residues of mutant E145G/Y227F and bound substrate (NAG)4
| Protein residues | Subsites for sugar moieties |
|---|---|
| Gly-145a, Glu-190 | +2 |
| Gln-109, E145Qb, Glu-190, Gln-225 | +1 |
| Gln-109a, Asp-143, E145Qb, Gln-225, Asn-228, Ala-287a, Trp-333 | −1 |
| Asn-45, Trp-333, Trp-337 | −2 |
a The main-chain interaction.
b The residue E145Q was referred from the complex structure of E145Q/Y227F+(NAG)2.
